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KMID : 1007520180270041157
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Food Science and Biotechnology
2018 Volume.27 No. 4 p.1157 ~ p.1163
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Preventive effect of Ephedra sinica extract on UVB-induced COX-2 and MMP-1 expression
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Ha Su-Jeong
Lee Chang-Joo
Jung Sung-Keun
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Abstract
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Ultraviolet B (UVB)-induced cyclooxygenase (COX)-2 and matrix metalloproteinase (MMP)-1 are representative markers for skin inflammation and photoaging, respectively. To evaluate compounds that may counteract the effects of UVB-induced skin damage, we developed an immortalized human keratinocyte (HaCaT) cell line with an MMP-1 reporter construct. Among the 30 botanical extracts screened, we selected Ephedra sinica extract (ESE) as a promising candidate and confirmed that ESE significantly suppresses UVB-induced COX-2 and MMP-1 expression in HaCaT cells. Treatment with ESE also potently suppressed UVB-induced ERK1/2 phosphorylation, as well as UVB-induced MEK1/2 and Raf phosphorylation in HaCaT cells. These findings suggest that our MMP-1 reporter system can be used to evaluate compounds with anti-inflammatory and anti-photoaging effects. We also report that ESE has potent suppressive effects against COX-2 and MMP-1 expression, which occurs via downregulation of Raf/MEK1/2/ERK1/2 phosphorylation.
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KEYWORD
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Cyclooxygenase-2, Ephedra sinica extract, Matrix metalloproteinase-1, Mitogen-activated protein kinase
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